From the Massachusetts Turnpike:

Take the Prudential/Copley Square Exit. Stay right when driving off the Copley Square exit. You will be on Stuart Street. Go through 5 sets of lights. At the 6 th set of lights turn left onto Charles Street. Cross Boylston Street (Traffic Light) and take your next right onto Beacon Street. Follow Beacon Street past the State House and continue down the hill until it merges into School Street (this will be after the traffic light at Tremont Street.) Take a left at the end of School Street. You will be on Washington Street. Our location is 250 Washington Street across from PI Alley and adjacent to Starbucks. A pay parking garage is available directly across the street.

From Southeast Expressway:

From the Expressway take the Downtown Boston exit. Cross Kneeland Street to Lincoln Street. At the lights on Service Road keep going straight to Summer Street. Cross Summer Street to Devonshire Street. At the first lights on Devonshire Street turn left onto Franklin Street and take the first sharp right at Arch Street. Arch Street will bring you to Milk Street. Take a left onto Milk Street. You will come to Washington Street. Turn right on to Washington Street. Our location is 250 Washington Street across from PI Alley and adjacent to Starbucks. A pay parking garage is available directly across the street.

From the MBTA:

The MBTA services Boston and the surrounding communities. Its services include a subway system, commuter rail trains and bus routes. Main DPH Building at 250 Washington Street, Boston is within a 5 minutes walk from the State Street MBTA station, which can be reached by the MBTA Orange Line. You can access the MBTA web site, which contains a general description, subway and commuter rail maps, and bus route searches, by clicking on the web site listed below the MBTA map.
This information is provided by the Department of Public Health.